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Lactose Intolerance, Breath Test

Includes 5 tests
15 Days

Lactose intolerance is a common condition characterized by the inability to properly digest lactose, a sugar in milk and dairy products. When individuals with lactose intolerance consume lactose-containing foods, they may experience gastrointestinal symptoms such as bloating, gas, abdominal pain, and diarrhea. The breath test for lactose intolerance, which measures hydrogen (H2) gas levels in breath samples, is a diagnostic tool to identify this condition and guide appropriate dietary modifications and treatment.

The breath test for lactose intolerance is based on the principle of bacterial fermentation of unabsorbed lactose in the colon. Usually, lactose is broken down by the enzyme lactase in the small intestine into glucose and galactose, which are absorbed into the bloodstream. In individuals with lactose intolerance, insufficient lactase enzyme activity leads to undigested lactose reaching the colon. Bacteria in the colon ferment the unabsorbed lactose, producing hydrogen gas as a byproduct. This excess hydrogen is absorbed into the bloodstream and exhaled through the lungs, where it can be detected and measured in breath samples.

See also myGenomiX® Lactose Intolerance, Genetic Testing

The lactose intolerance breath test typically involves the following steps:

  • Preparation: Patients are instructed to follow specific dietary restrictions for a period leading up to the test to minimize the presence of other fermentable substances that could confound the results. This may include avoiding dairy products and lactose-containing foods for a specified period.
  • Baseline Breath Sample: A baseline breath sample is collected to establish the patient's baseline hydrogen levels before ingesting the lactose solution.
  • Lactose Ingestion: The patient drinks a standardized solution containing a predetermined amount of lactose.
  • Breath Sampling: Breath samples are collected at regular intervals, typically every 30-60 minutes, for 3 hours. These samples are analyzed for hydrogen gas concentrations using a breath analyzer.
  • Interpretation: An increase in hydrogen levels above the baseline measurement indicates bacterial fermentation of lactose in the colon, confirming lactose intolerance.
Clinical Significance

The lactose intolerance breath test is valuable in diagnosing lactose intolerance and differentiating it from other gastrointestinal disorders with similar symptoms. Identifying lactose intolerance allows healthcare providers to recommend dietary modifications to minimize symptoms and improve the quality of life for affected individuals. It also helps avoid unnecessary treatments and interventions for conditions that may mimic lactose intolerance.

Management Strategies

Once lactose intolerance is diagnosed, management strategies typically focus on dietary modifications and symptom management. Recommendations may include:

  • Dietary Avoidance: Patients are advised to limit or avoid lactose-containing foods and beverages, such as milk, cheese, yogurt, and ice cream. Choosing lactose-free or lactose-reduced alternatives can help minimize symptoms.
  • Lactase Supplements: Over-the-counter lactase enzyme supplements may be recommended to help individuals with lactose intolerance digest lactose-containing foods more effectively. These supplements can be taken with meals to reduce symptoms.
  • Nutritional Counseling: Working with a registered dietitian can help patients identify and navigate lactose-containing foods and develop a well-balanced diet that meets their nutritional needs while minimizing symptoms.
  • Symptom Management: Over-the-counter medications or dietary supplements may be recommended to help alleviate gastrointestinal symptoms such as bloating, gas, and diarrhea.
  • Monitoring and Follow-Up: Regular follow-up appointments with healthcare providers are essential to monitor symptom progression, assess dietary adherence, and adjust the management plan.

The breath test for lactose intolerance is a valuable diagnostic tool that helps identify individuals who cannot properly digest lactose, a common dietary component. By accurately diagnosing lactose intolerance, healthcare providers can implement targeted dietary modifications and symptom management strategies to improve the quality of life for affected individuals.

Additional information
Tests includedIncludes 5 tests
Sample Other
Procedure completion test
Step 1

Purchase the test you want online

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Step 2


We send you the certified package - sampling collection kit, to collect your sample, always in accordance with the instructions contained within the kit.

Step 3

Sending your sample

After you have collected your sample, place it in the prepaid shipping package, contact the courier company and send it to our certified laboratory.

Step 4

Receiving the test results

Download your test results easily and securely anytime you want by logging in to your personal account.

Step 1

Book an appointment and buy the test online

Select from the most complete range test of Prevention, Andrology and Diagnostics, book an appointment in real time and purchase them online.

Step 2


Visit the certified laboratory of Diagnostiki Athinon on the date and time you have chosen, to perform the sampling.

Step 3

Receiving the test results

Download your test results easily and securely anytime you want by logging in to your personal account.

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